EmotionaI Intelligence (EI), Management, and Leadership-Terence Essay

Transformation of e.coli with plasmid dna and ligated dna Essay

Change of e.coli with plasmid dna and ligated dna - Essay Example nce quality on the plasmid fills the need of a selectable marker that gives another trademark to the changed cell not controlled by the non-changed cell, consequently recognizing from the non-transformant on a specific media. The ‘No DNA’ control on the LB plate (which contains no anti-microbial or X-lady) is relied upon to show grass example of bacterial development inferring that the bacterial cells are reasonable and can develop without the anti-infection ampicillin. Anyway the No DNA on the LB blue plate is relied upon to show no development as they don't contain the important quality for the limitation catalyst to make due in the medium with ampicillin. Cylinder 2 shows every single blue province which represents that change of cells with plasmid pCK103 meets the bacterial cells with the capacity to develop in ampicillin medium because of the nearness of the opposition quality and furthermore convert X-lady in the medium to create blue states by the activity of lac Z quality present in the plasmid which encodes ï  ¢-galactosidase engaged with lactose digestion. 3). x ï  ­g of the plasmid pCK103 were added to the E. coli during the change. From your outcomes ascertain the complete number of transformants created by this measure of DNA. Recollect that the 100ï  ­l example is just 50% of the absolute change and the 10ï  ­l example is, obviously, a twentieth. 4). This worth is known as the change recurrence and is a proportion of the effectiveness of the procedure. It is somewhat subject to the plasmid utilized however for pCK103 an estimation of 106 - 107 transformants per ï  ­g might be normal under perfect conditions. How does your worth contrast with this? For what reason do you think it varies? The worth contrasts from the perfect change effectiveness anticipated for pCK103. Various elements influence change proficiency, for example, the real DNA fixation and measure of DNA, heat stun, timeframe for articulation after change just as the specific plates utilized. The outcomes

The Best New Years Career Resolutions for People in Their 30s

The Best New Years Career Resolutions for People in Their 30s When you think of a year having 365 days, or 52 weeks, you would think that you have ample time to achieve your goals.Add to that the life expectancy in the US of 78.6 years and you have lots of time. Right?If you have all that timeâ€"a lot of time indeedâ€"how come you don’t get to achieve those things you set your heart upon? How come the end of the year comes upon you so suddenly like a thief?Is it a lack of planning? Is it the lack of good goals, smart goals or realistic goals?And if so, then which goals are realistic? And just who gets to decide this anyway?The fact that some people have achieved what the majority think is impossible is evidence that goals are individual.For that reason, a “realistic” goal is only as realistic as you determine it to be.Consider the below examples of people who beat all odds to achieve what they wanted in life.Stephen Hawking beat disabilities and battled Amyotrophic Lateral Sclerosis to continue with research and teaching using electronic devices. He had to train his mind to do things differently.Marla Runyan, legally blind from childhood, never stopped education. And when she joined sports, she came out with 5 gold medals for the Paralympics, has run in the Olympics and several marathons.Dominic O’Brien became an 8-time World Memory Champion with a Guinness World Record for memorizing the order of 54 shuffled packs of playing cards (2,808 cards) after viewing them just once. He also got the Brain of the Year and the Grand Master of Memory awards.Soichiro Honda left home at 15, worked as an apprentice for 6 years then started his own auto repair business at 22. Forming the Honda Motor Company while 42, it only took him 10 years for his company to become a leading motorcycle manufacturer in the world.Aren’t these the kind of people who motivate you? Aren’t their stories the ones which make you want to strip your life of everything and run towards your goal with 100% focus?But somehow, you don’t do that.Many re asons can be given for that. Maybe some of them are excuses.The Need for Goals in LifeDespite all the different philosophies and theories, one fact stands unopposed. You must have goals in life.This is not for the sake of fitting in. Many people have new year’s resolutions but few achieve them. So get out of the mob psychology and have a solid reason as to why you need new year’s resolutions.Life is a journey and never a destination.That means that you should not only think of life in terms of where you are going, but also how you get there. What you go through to get there.The challenges and victories. These are important and they are what define life.A failure to appreciate the journey makes you lose the strength to get to the destination.This is why you may be giving up so often because you face challenges.The problem is that you expect not to face challenges. Yet those challenges are what make you the right person for the destination. Have you ever noticed that only a certai n kind of people are in certain places?Look at successful business people. If you look into their lives, you’ll notice that they have some similarities. Yes, they have their differences but some things are common across the board.Look into the private lives of successful sportsmen and women. Ignore the scandals some of them are faced with. Do you know that they develop themselves for the success?That development comes through appreciating the journey. They embrace the challenges and work through them.So, the journey is important. But the destination is also important.Why?Because it is what determines the journey you take.The destination determines the path you take. If going to the coffee shop, you can walk.But if going to another continent, you fly.Those are two different journeys and they are determined by the destination.NEW YEAR CAREER RESOLUTIONSIn making new year’s resolutions, decide one thing this time round. You will embrace the journey. You will fight to get to the des tination but will also appreciate everything that comes your way.As you make your very personal career resolutions, here are some foundational ones to consider. These are not necessarily telling you how to make your career better.They are telling you the ingredients to ensuring the resolutions you make work.As you go through these, you’ll see how they tie in with the rest of your goals.Learn from the PastBeing in your 30s is very different from being in your 20s. You are more than a decade older now. Congratulations.So, are you better than you had been in the past decade? If you started working at 25, how have you progressed this far? Can you pinpoint specific moments of victory and loss?One of the most important things about progress is learning. You can never outgrow learning. Even in old age, with gray hair, there will still be something to learn.You can however stop learning if you decide there is no need for it. Fortunately, you haven’t gotten to that point, otherwise you w ouldn’t be reading this.To learn from the past, consider the below:1. What workedYou have definitely experienced victories in your life this far. But did you take time to find out just how you attained those successes? Did you think about the processes you followed? Did you give thought to the challenges you faced and how you overcame them?This is the right time to do this in case you didn’t. You see, in your 30s, you have tried what you first believed and now have an opportunity to make appropriate changes. If you noticed some methods or tools working well, then stick to those.But as you do, try and improve them. See where they fall short and strengthen them accordingly. Because if you are serious about achieving your life goals, more challenges lie ahead.2. What didn’t workFrom all the things you tried and have been trying, you must have noticed that some just never work. Maybe you’ve been using methods someone told you worked for them, even though they don’t work for yo u. It might be time to drop them.When you try fitting yourself into something which isn’t really fitting, you are actually working towards your own failure.The biggest thing you are risking is your confidence levels. You are trying to please the person by applying his advice even though it’s not working.Don’t waste any more time. Look for what works for you and embrace it.You can ask more people how they do things. You can check online for opinions and reviews.Better still, you can utilize your creativity and come up with unique ways of getting ahead.Work on Your Financial DisciplineMoney is not everything. It is not the measure of success. Noble examples like Mother Teresa prove this.But that doesn’t mean that money is not important. In fact, anyone claiming that money is not important will be lying. No-one wants to live in poverty. And yes, poverty is not the lack of money. It is mainly a mentality issue.But there is more to money that this.Diving slightly deeper, money do es more than buying food. It does more than buying a good home and obviously, it takes care of many problems. Source: PersonalFNFor career purposes, money is necessary for further schooling. It is necessary for dressing like the professional you are, or intend to be. It is necessary for networking in certain circles. This list can be long, but you get the idea.Yet money is not self-managing. It doesn’t have the ability to teach you how best to use it. Unless you use it to learn how to use it.More than that, you just need to mismanage your finances to see what trouble you can get into.Financial impropriety at a personal level can be career wrecking. The levels of stress resulting from it won’t help but show up in the office.If you are struggling to foot your bills, you will not be able to concentrate at work. Meaning you won’t be productive at all due to high stress levels.Is there any way your career will grow in such an environment? Very unlikely.Compare that to the confidence levels you gain when you know that you are not in debt. When you freely help out friends when they are in nee d.When you know that being fired can’t scare you since you have big savings. When you get email updates about the growth of your investments.This confidence is great for a boost in career success.Check your expenses. Have some savings and invest some money. Get yourself covered and see the peace you have while chasing your dreams.Find a MentorAre you humble enough to learn from others?It’s said that experience is the best teacher. But if you are keen enough, you’ll realize that this is not always true.When you make mistakes in your investments and lose your money, is this the best way for you to learn how to invest?No.It would have been better if someone walked you through the dos and don’ts and told you how to go about it.If that happened, then maybe you needed someone who would go the extra mile to teach you what to look for in an investment option.Maybe which investment vehicles are best for your situation.Now, finding such people is not easy. And though you may know some , reaching them might be your biggest problem.You still have to get through the fears of asking them to mentor you.Because the truth is that not every expert is open to mentoring others.So what do you do?Here are two ideas you can try:1. Approach them from a business perspectiveOne thing common with all experts is that they worked hard to become who they are. As such, you cannot get them to work with you if you don’t show value for their expertise.The reason people pay for mentorship is that they understand the value. They are willing to pay for personal guidance and since it cost them, they apply it.Often, what costs you nothing carries very little value to you. So when you approach a potential mentor from this perspective, he sees your need for help. You can be sure you’ll get more than you pay for if you are proactive.2. Learn from them behind the scenesOf course, not everyone who can’t afford conferences is simply not valuing good advice. You might understand the value of what you want but can’t prove it in terms of money at the moment.If this is you, cheer up. Where there is a will, there is a way. But there is still a cost involved, albeit less than the above option calls for.Get to the internet and search up the mentor of your choice. Get to his website and read the material he has there. Check him out on YouTube and follow him on social media. Experts always give tips and so much insight even in tweets. This is because they are full of the knowledge so it just flows out of them.This is what you will harvest. Since you understand the value, you will put it to use.Another avenue for gaining insight from your mentor is by reading his books. Books contain more than what someone can share on a platform.From a book, you can learn the challenges your mentor went through and be encouraged.So, if by some chance you can’t get your favorite mentor to physically walk with you, do it virtually. This is how many experts themselves grow.They learn from each other through many means, including reading their books.Work on Your HealthYou have your eyes set on improving your career. But have you considered improving your health?Do not ignore your health. It is your body that works. It is your mind that thinks through problems and comes up with solutions.It is your eyes which help you read reports and emails. It is your heart that distributes nutrients throughout your body using pumped blood.Can you really afford to neglect the body that does all these and many other things? The answer is no. Here are three things to work on:1. FoodThere has been lots of talk about healthy eating. And there is need for more talk about it. But more importantly, there is need for people to start applying the information they gather from the talks and seminars.Eating healthy is not difficult. It just needs some dedication. Dedication to do away with white rice and embrace brown rice. Avoid white bread and take brown or wholemeal.As far as grains are concerned , just do away with anything white and embrace brown, or even better, whole.Taking fruits and increasing your vegetable intake is quite obvious. Here is a list of nutrients found in popular vegetables. And here is one for popular fruits.Do not ignore the need to take proteins. If conscious of your weight, which is a good thing, go lean. Use steak instead of the usual beef because the fat in it is not good.Take the healthy fat from oily fish and get better eyes and brain cells.2. WaterIf water making up to 60% of your body doesn’t tell you its importance, then you need help. Dehydration affects your brain’s functioning and your muscles too.Also of importance here is the fact that water helps detoxify your body. This body which works so hard to get you to your goals, has some things it needs to get rid of. Without water, your excretory system will not function well enough. Source: USGSNot taking water is an easy way of slowing yourself down. Yet you’re expecting to achieve your new year’s resolutions.3. ExerciseIgnore the toned body and the protruding muscles which serve as proof of how strong you are. Do you know there are more benefits to exercising than these?Do not focus on muscles only. For wholesome success, you have to gain the inward benefits of exercising too.These happen in the mind, heart and yes, body muscles. When your heart is strong, it does its job well. Commit to improving the strength and overall health of your heart through cardiac exercises.Your mind will need mental exercises while your body will do well with physical exercise.Still, physical workout benefits both your heart and mind, even as your muscles gain from it.Divide Your Goals Into Short And Long TermYour goals should be big. In fact, they should be so big that they are scary. If they are not, then you may not be someone worth remembering.Look at the people mentioned a t the beginning of this article. They have achieved serious goals and that has given them much popularity.We are not telling you to seek popularity. We are just pointing out that the reason these people are popular is the kind of goals they achieved.But as good and admirable as big goals and dreams are, they cannot be achieved as they are. This is one of the reasons many people don’t move forward.They listen to successful people talk and want to be like them.Then they develop a desire to succeed quickly. They want big results fast.This is why at the beginning, we told you that you have to anticipate and appreciate the journey.Your big goals need to be broken down in order to be worked on.For example, your big goal may be to have the biggest online marketing business in the country. You will never achieve this goal as it is.This is because there is so much to be done to get there.You may not even understand half of what needs to be done, yet it has to be done.For this big goal, you need to have smaller onesâ€"your short-term goals.These can include building a website, building an online presence through social media, market research, learning copywriting or hiring copywriters etc.CONCLUSIONYou have no excuse to lag behind.Whether you already made your new year’s resolutions or want to do it now, pick something from here.Implement it alongside the resolutions you come up with. In so doing, you will ensure that before you hit 40, you have much to be proud of.

Facts, Geography About the West African Nation of Ghana

Ghana is a country located in western Africa on the Gulf of Guinea. The country is known for being the second largest producer of cocoa in the world as well as its incredible ethnic diversity. Ghana currently has more than 100 different ethnic groups in its population of just over 24 million. Fast Facts: Ghana Official Name: Republic of GhanaCapital: AccraPopulation: 28,102,471Â  (2018)Official Language: EnglishCurrency: Cedi (GHC)Form of Government: Presidential republicClimate: Tropical; warm and comparatively dry along southeast coast; hot and humid in southwest; hot and dry in northTotal Area: 92,098 square miles (238,533 square kilometers)Highest Point: Mount Afadjato at 2,904 feet (885 meters)Lowest Point: Atlantic Ocean at 0 feet (0 meters) History of Ghana Ghanas history prior to the 15th century is concentrated primarily on oral traditions. However, it is believed that people may have inhabited what is present-day Ghana from about 1500 BCE. European contact with Ghana began in 1470. In 1482, the Portuguese built a trading settlement there. Shortly thereafter for three centuries, the Portuguese, English, Dutch, Danes, and Germans all controlled different parts of the coast. In 1821, the British took control of all of the trading posts located on the Gold Coast. From 1826 to 1900, the British then fought battles against the native Ashanti and in 1902, the British defeated them and claimed the northern part of todays Ghana. In 1957, after a plebiscite in 1956, the United Nations determined that the territory of Ghana would become independent and combined with another British territory, British Togoland, when the entire Gold Coast became independent. On March 6, 1957, Ghana became independent after the British gave up control of the Gold Coast and the Ashanti, the Northern Territories Protectorate and British Togoland. Ghana was then taken as the legal name for the Gold Coast after it was combined with British Togoland in that year. Following its independence, Ghana underwent several reorganizations that caused the country to be divided into 10 different regions. Kwame Nkrumah was the first prime minister and president of modern Ghana and he had goals of unifying Africa as well as freedom and justice and equality in education for all. His government, however, was overthrown in 1966. Instability was then a major part of Ghanas government from 1966 to 1981, as several government overthrows occurred. In 1981, Ghanas constitution was suspended and political parties were banned. This later caused the countrys economy to decline and many people from Ghana migrated to other countries.By 1992, a new constitution was adopted, the government began to regain stability, and the economy started to improve. Today, Ghanas government is relatively stable and its economy is growing. Government of Ghana Ghanas government today is considered a constitutional democracy with an executive branch made up of a chief of state and a head of government filled by the same person. The legislative branch is a unicameral Parliament while its judicial branch is made up of the Supreme Court. Ghana is also still divided into 10 regions for local administration: Ashanti, Brong-Ahafo, Central, Eastern, Greater Accra, Northern, Upper East, Upper West, Volta, and Western. Economics and Land Use in Ghana Ghana currently has one of the strongest economies of West Africas countries due to its richness of natural resources. These include gold, timber, industrial diamonds, bauxite, manganese, fish, rubber, hydropower, petroleum, silver, salt, and limestone. However, Ghana remains dependent on international and technical assistance for its continued growth. The country also has an agriculture market that produces things like cocoa, rice, and peanuts, while its industries are focused on mining, lumber, food processing, and light manufacturing. Geography and Climate of Ghana Ghanas topography consists mainly of low plains but its south-central area does have a small plateau. Ghana is also home to Lake Volta, the worlds largest artificial lake. Because Ghana is only a few degrees north of the Equator, its climate is considered tropical. It has a wet and dry season but it is mainly warm and dry in the southeast, hot and humid in the southwest and hot and dry in the north. More Facts About Ghana Bordering Countries: Burkina Faso, Cote dIvoire, TogoCoastline: 335 miles (539 km)Ghana has 47 local languages.Association football or soccer is the most popular sport in Ghana and the country regularly participates in the World Cup.Ghanas life expectancy is 59 years for males and 60 years for females. Sources Central Intelligence Agency. CIA - The World Factbook - Ghana. United States Department of State. ï » ¿Ghana.Infoplease. Ghana: History, Geography, Government, and Culture.

What Makes A Look Fashionable - 1484 Words

With this in mind, it’s also important to remember that although consumers play a major role in selecting styles, it’s fashion experts that are what truly makes a look fashionable in the first place (Farennikova and Prinz 98). This brings us to our next fashion condition which is that â€Å"individuals regarded as experts, though not necessarily fashion professionals, must endorse the look† (Farennikova and Prinz 104). This simply refers to how an expert, using their specific taste, will pinpoint and predict a certain trend that is then regarded in the media as fashionable due to the mere mention of it by these particular people of power. They can range from a public figure like Kate Moss to a familiar fashion figure such as Anna Wintour who,†¦show more content†¦This being said, this expertise all depends on the level of fashion being compared originally; whether it’s anything from High Fashion to Haute Couture, the absurdly priced and impeccably constructed fashion which is by no means intended for the average wearer. Many of these pieces are mere exaggerations of the possible styles to arise from them (with their actually use being to remain on the runway and kept separate from the consumers), this fashion is different then mass-fashion which is something that experts do not have a say in regardless of the occasion. â€Å"In mass-fashion, they will tell us to take the trend to the streets; in High Fashion, the command is to worship.† (Farennikova and Prinz 100-101). This can be said for the Spring 2016 Gucci Collection which is both immaculately crafted and likely astronomically priced if its availability is an option at all. Unless you near future involves plans of wearing a magenta lace neck-ruff paired with a matching pink floral suit and blue sneakers in the coming seasons, it is unlikely you’ll be banging down doors in order to acquire this distinct look. This is why mass-fashion is selected and flouris hes independently from experts, but they’re still the leading cause as to how you ended up with that eclectic style or look in the first place (Farennikova and Prinz 100). As a side note, this can also lead to the topic of â€Å"timeless fashion† and whether or

Economic Immigration Free Essays

Chavez Department of Chicana and Chicano Studies at the University of California, Los Angeles. He has a Ph. D. We will write a custom essay sample on Economic Immigration or any similar topic only for you Order Now n political science from the University of Chicago. The thesis statement or main argument of this article is the following, â€Å"Comprehensive immigration reform that legalizes currently unauthorized immigrants and creates flexible legal limits on future immigration in the context of full labor rights would help American workers and the U. S economy. † Clearly the main goal of this Dr. OJeda writing this paper is shed light on the many economic benefits for the United States if a comprehensive immigration reformation law was passed. Some of the evidence used by the author to validate the his thesis is for xample this excerpt from the article, â€Å"The historical experience of legalization under the 1986 Immigration Reform and Control Act indicates that comprehensive immigration reform would raise wages, increase consumption, create Jobs and generate additional tax revenue. Even though the Immigration Reform and Control Act was implemented during a period that included a recession and high unemployment (1990-1991), it still helped raise wages and spurred increases in educational, home, and small business investments by newly legalized immigrants. Taking the experience of the Immigration Reform and Control Act as a starting point, e estimate that comprehensive immigration reform would yield at least $1. 5 trillion in added U. S gross domestic product (GDP) over 10 years. † (Ojeda, 2012, 176) I am an immigrant from Mexico myself and I know the struggle that goes on each and every day in the lives of illegal immigrants. I moved here when I was 4 years old and have been raised in a Mexican-American culture. I love this country and consider myself an American, this country has provided for me the path to live out my dreams and be whatever I choose to be in life, and for that I will always be grateful. Just last ear thanks to the Deferred Action law that was put into effect, more than 700,000 undocumented students qualified for a 2 year work/study permit, a chance to get a driver’s license and to finally have legal standing and documentation in this country. The effect it will have on the future of the United States is a positive one and will benefit the growth of the country in many sectors greatly. I strongly believe this article is crucial to the United States we live in today and to the future of the United States. It exposes a lot of myths and calms a lot of worries concerning whether or not comprehensive immigration reform would help the country at all. How to cite Economic Immigration, Papers

Introduction Pollution Research Paper Example

Introduction Pollution Paper Pollution is the introduction oaf contaminant into a natural environment, usually by humans. While most people think of pollution as chemical waste dumped into rivers, or factories spewing toxins into the air, it can also include light pollution or sound pollution. These are example of pollutions such as air pollution, water pollution and land pollution. Firstly, the air pollution is the accumulation in the atmosphere of substances that, in sufficient concentrations, endanger human health or produce other measured effects on living matter and other materials. Among the major sources of pollution are power and heat generation, the burning of solid wastes, industrial processes, and, especially, transportation. The six major types Of pollutants are carbon monoxide, hydrocarbons, nitrogen oxides, particulates, sulfur dioxide, and photochemical oxidants. These are examples of air pollution; * Tobacco Smoke Tobacco smoke is one of the major forms of pollution in buildings. It is not only the smoker who is infected, but everyone who inhales the polluted air. There is a very strong connection between smoking and lung cancer. Bronchitis is common among smokers and unborn babies of mothers who smoke also suffer from the harmful effects of smoking. * Exhaust Gases of Vehicles Pollution from exhaust gases of vehicles is responsible for 60% of all air pollution and in cities up to 80%. There is a large variety of harmful chemicals present in these gases, with lead being one Of the most dangerous. Combustion of Coal The combustion of coal without special precautions can have serious consequences. We will write a custom essay sample on Introduction Pollution specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Introduction Pollution specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Introduction Pollution specifically for you FOR ONLY $16.38 $13.9/page Hire Writer If winds do not blow away the poisonous gases, they can have fatal effects and may lead to death. Acid rain Acid rain is the term for pollution caused when sulfur and nitrogen dioxides combine with atmospheric moisture to produce highly acidic rain, snow, hail, or fog. The acid eats into the stone, brick and metal articles and pollutes water sources. Coal in South Africa is rich in sulfur and the power stations in the Mulligan Province could be responsible for acid rain over other areas of our country. Moreover, another example of pollution is water pollution. Water pollution is the introduction into fresh or ocean waters of chemical, physical, or illogical material that degrades the quality of the water and affects the organisms living in it. This process ranges from simple addition of dissolved or suspended solids to discharge of the most insidious and persistent toxic pollutants (such as pesticides, heavy metals, and no degradable, bio accumulative, chemical compounds). These are examples Of water pollution * Industrial affluent Water is discharged from after having been used in production processes. This waste water may contain acids, alkalis, salts, poisons, oils and in some cases harmful bacteria. K Mining and Agricultural Wastes Mines, especially gold and coal mines, are responsible for large quantities of acid water. Agricultural pesticides, fertilizers and herbicides may wash into rivers and stagnant water bodies. Sewage Disposal and Domestic Wastes Sewage as well as domestic and farm wastes were often allowed to pollute rivers and dams. Lastly is the land pollution. Land pollution is the degradation of the Earths land surface through misuse of the soil by poor agricultural practices, mineral exploitation, industrial waste dumping and indiscriminate disposal of urban wastes. It includes visible waste and litter as well as pollution of the soil itself. Examples of Land Pollution; * Soil Pollution Soil pollution is mainly due to chemicals in herbicides (weed killers) and pesticides (poisons which kill insects and other invertebrate pests). Litter is waste material dumped in public places such as streets, parks, picnic areas, at bus stops and near shops. * Waste Disposal The accumulation Of Waste threatens the health Of people in residential areas. Waste decays, encourages household pests and turns urban areas into unsightly, dirty and unhealthy places to live in.